Ontology highlight
ABSTRACT:
SUBMITTER: King MR
PROVIDER: S-EPMC6959270 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
King Matthew R MR Petry Sabine S
Nature communications 20200114 1
Phase separation of substrates and effectors is proposed to enhance biological reaction rates and efficiency. Targeting protein for Xklp2 (TPX2) is an effector of branching microtubule nucleation in spindles and functions with the substrate tubulin by an unknown mechanism. Here we show that TPX2 phase separates into a co-condensate with tubulin, which mediates microtubule nucleation in vitro and in isolated cytosol. TPX2-tubulin co-condensation preferentially occurs on pre-existing microtubules, ...[more]