Ontology highlight
ABSTRACT:
SUBMITTER: Kwon J
PROVIDER: S-EPMC6959352 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Kwon Jiwoong J Park Jong-Seok JS Kang Minsu M Choi Soobin S Park Jumi J Kim Gyeong Tae GT Lee Changwook C Cha Sangwon S Rhee Hyun-Woo HW Shim Sang-Hee SH
Nature communications 20200114 1
We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. UnaG only fluoresces upon binding of a fluorogenic metabolite, bilirubin, enabling UV-free reversible photoswitching with easily controllable kinetics and low background under Epi illumination. The on- and off-switching rates are controlled by the concentration of the ligand and the excitation light i ...[more]