Dataset Information

Uptake, tissue distribution, and toxicity of polystyrene nanoparticles in developing zebrafish (Danio rerio).

ABSTRACT: Plastic pollution is a critical environmental concern and comprises the majority of anthropogenic debris in the ocean, including macro, micro, and likely nanoscale (less than 100nm in at least one dimension) plastic particles. While the toxicity of macroplastics and microplastics is relatively well studied, the toxicity of nanoplastics is largely uncharacterized. Here, fluorescent polystyrene nanoparticles (PS NPs) were used to investigate the potential toxicity of nanoplastics in developing zebrafish (Danio rerio), as well as to characterize the uptake and distribution of the particles within embryos and larvae. Zebrafish embryos at 6h post-fertilization (hpf) were exposed to PS NPs (0.1, 1, or 10ppm) until 120 hpf. Our results demonstrate that PS NPs accumulated in the yolk sac as early as 24 hpf and migrated to the gastrointestinal tract, gallbladder, liver, pancreas, heart, and brain throughout development (48-120 hpf). Accumulation of PS NPs decreased during the depuration phase (120-168 hpf) in all organs, but at a slower rate in the pancreas and gastrointestinal tract. Notably, exposure to PS NPs did not induce significant mortality, deformities, or changes to mitochondrial bioenergetics, but did decrease the heart rate. Lastly, exposure to PS NPs altered larval behavior as evidenced by swimming hypoactivity in exposed larvae. Taken together, these data suggest that at least some nanoplastics can penetrate the chorion of developing zebrafish, accumulate in the tissues, and affect physiology and behavior, potentially affecting organismal fitness in contaminated aquatic ecosystems.

SUBMITTER: Pitt JA

PROVIDER: S-EPMC6959514 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Uptake, tissue distribution, and toxicity of polystyrene nanoparticles in developing zebrafish (Danio rerio).

Pitt Jordan A JA Kozal Jordan S JS Jayasundara Nishad N Massarsky Andrey A Trevisan Rafael R Geitner Nick N Wiesner Mark M Levin Edward D ED Di Giulio Richard T RT

Aquatic toxicology (Amsterdam, Netherlands) 20171124

Plastic pollution is a critical environmental concern and comprises the majority of anthropogenic debris in the ocean, including macro, micro, and likely nanoscale (less than 100nm in at least one dimension) plastic particles. While the toxicity of macroplastics and microplastics is relatively well studied, the toxicity of nanoplastics is largely uncharacterized. Here, fluorescent polystyrene nanoparticles (PS NPs) were used to investigate the potential toxicity of nanoplastics in developing zeb ...[more]

PMID: 29197232

Similar Datasets

Project description:Glutathione (GSH), the most abundant vertebrate endogenous redox buffer, plays key roles in organogenesis and embryonic development, however, organ-specific GSH utilization during development remains understudied. Monochlorobimane (MCB), a dye conjugated with GSH by glutathione-s-transferase (GST) to form a fluorescent adduct, was used to visualize organ-specific GSH utilization in live developing zebrafish (Danio rerio) embryos. Embryos were incubated in 20 μM MCB for 1 h and imaged on an epifluorescence microscope. GSH conjugation with MCB was high during early organogenesis, decreasing as embryos aged. The heart had fluorescence 21-fold above autofluorescence at 24 hpf, dropping to 8.5-fold by 48 hpf; this increased again by 72 hpf to 23.5-fold, and stayed high till 96 hpf (18-fold). The brain had lower fluorescence (10-fold) at 24 and 48 hpf, steadily increasing to 30-fold by 96 hpf. The sensitivity and specificity of MCB staining was then tested with known GSH modulators. A 10-min treatment at 48 hpf with 750 μM tert-butylhydroperoxide, caused organ-specific reductions in staining, with the heart losing 30% fluorescence, and, the brain ventricle losing 47% fluorescence. A 24 h treatment from 24-48 hpf with 100 μM of N-Acetylcysteine (NAC) resulted in significantly increased fluorescence, with the brain ventricle and heart showing 312% and 240% increases respectively, these were abolished upon co-treatment with 5 μM BSO, an inhibitor of the enzyme that utilizes NAC to synthesize GSH. A 60 min 100 μM treatment with ethacrynic acid, a specific GST inhibitor, caused 30% reduction in fluorescence across all measured structures. MCB staining was then applied to test for GSH disruptions caused by the toxicants perfluorooctanesulfonic acid and mono-(2-ethyl-hexyl)phthalate; MCB fluorescence responded in a dose, structure and age-dependent manner. MCB staining is a robust, sensitive method to detect spatiotemporal changes in GSH utilization, and, can be applied to identify sensitive target tissues of toxicants.

Dataset Information

Uptake, tissue distribution, and toxicity of polystyrene nanoparticles in developing zebrafish (Danio rerio).

Publications

Uptake, tissue distribution, and toxicity of polystyrene nanoparticles in developing zebrafish (Danio rerio).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets