Ontology highlight
ABSTRACT:
SUBMITTER: Lau E
PROVIDER: S-EPMC6961840 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Lau Edward E Han Yu Y Williams Damon R DR Thomas Cody T CT Shrestha Rajani R Wu Joseph C JC Lam Maggie P Y MPY
Cell reports 20191201 11
The protein-level translational status and function of many alternative splicing events remain poorly understood. We use an RNA sequencing (RNA-seq)-guided proteomics method to identify protein alternative splicing isoforms in the human proteome by constructing tissue-specific protein databases that prioritize transcript splice junction pairs with high translational potential. Using the custom databases to reanalyze ∼80 million mass spectra in public proteomics datasets, we identify more than 1, ...[more]