Targeting the tumor vasculature with engineered cystine-knot miniproteins.
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ABSTRACT:
SUBMITTER: Lui BG
PROVIDER: S-EPMC6962393 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Publications
Targeting the tumor vasculature with engineered cystine-knot miniproteins.
Lui Bonny Gaby BG Salomon Nadja N Wüstehube-Lausch Joycelyn J Daneschdar Matin M Schmoldt Hans-Ulrich HU Türeci Özlem Ö Sahin Ugur U
Nature communications 20200115 1
The extra domain B splice variant (EDB) of human fibronectin selectively expressed in the tumor vasculature is an attractive target for cancer imaging and therapy. Here, we describe the generation and characterization of EDB-specific optical imaging probes. By screening combinatorial cystine-knot miniprotein libraries with phage display technology we discover exquisitely EDB-specific ligands that share a distinctive motif. Probes with a binding constant in the picomolar range are generated by ch ...[more]
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