β-catenin inhibitors suppress cells proliferation and promote cells apoptosis in PC9 lung cancer stem cells.

ABSTRACT: This study aimed to investigate the effect of β-catenin inhibitors on cells proliferation and apoptosis in lung cancer stem cells (LCSCs). Drug-resistance PC9 cells were induced by escalation of cisplatin repeated treatment, and then PC9 LCSCs were constructed by Sphere Formation methods. Membrane expression of OCT4, SOX2, CD44, CD133 and β-Catenin were detected by Immunofluorescent staining, and mRNA of CSCs marker genes and Wnt/β-Catenin target genes were determined by qPCR assay. PC9 LCSCs were nurtured for 5 days (Day 5) and then β-catenin inhibitor pyrvinium pamoate (PP) with IC50 concentration (0.221 µM) and ICG-100 with IC50 concentration (2.620 µM) were added and cultured for another 2 days (Day 7), respectively. CCK8 and AV/PI assays were performed to detect cells proliferation an

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6966044 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature