Dataset Information

Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids.

ABSTRACT: Background:Omega-3 fatty acids have various health benefits in combating against neurological problems, cancers, cardiac problems and hypertriglyceridemia. The main dietary omega-3 fatty acids are obtained from marine fish. Due to the pollution of marine environment, recently microalgae are considered as the promising source for the omega-3 fatty acid production. However, the demand and high production cost associated with microalgal biomass make it necessary to implement novel strategies in improving the biomass and omega-3 fatty acids from microalgae. Results:Four plant hormones zeatin, indole acetic acid (IAA), gibberellic acid (GBA) and abscisic acid (ABA) were investigated for their effect on the production of biomass and lipid in isolated Chlorella sp. The cells showed an increase of the biomass and lipid content after treatments with the plant hormones where the highest stimulatory effect was observed in ABA-treated cells. On the other hand, IAA showed the highest stimulatory effect on the omega-3 fatty acids content, eicosapentaenoic acid (EPA) (23.25%) and docosahexaenoic acid (DHA) (26.06%). On the other hand, cells treated with ABA had highest lipid content suitable for the biodiesel applications. The determination of ROS markers, antioxidant enzymes, and fatty acid biosynthesis genes after plant hormones treatment helped elucidate the mechanism underlying the improvement in biomass, lipid content and omega-3 fatty acids. All four plant hormones upregulated the fatty acid biosynthesis genes, whereas IAA particularly increased omega-3-fatty acids as a result of the upregulation of omega-3 fatty acid desaturase. Conclusions:The contents of omega-3 fatty acids, the clinically important compounds, were considerably improved in IAA-treated cells. The highest lipid content obtained from ABA-treated biomass can be used for biodiesel application according to its biodiesel properties. The EPA and DHA enriched ethyl esters are an approved form of omega-3 fatty acids by US Food and Drug Administration (FDA) which can be utilized as the therapeutic treatment for the severe hypertriglyceridemia.

SUBMITTER: Sivaramakrishnan R

PROVIDER: S-EPMC6966795 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Plant hormone induced enrichment of <i>Chlorella</i> sp. omega-3 fatty acids.

Sivaramakrishnan Ramachandran R Incharoensakdi Aran A

Biotechnology for biofuels 20200117

<h4>Background</h4>Omega-3 fatty acids have various health benefits in combating against neurological problems, cancers, cardiac problems and hypertriglyceridemia. The main dietary omega-3 fatty acids are obtained from marine fish. Due to the pollution of marine environment, recently microalgae are considered as the promising source for the omega-3 fatty acid production. However, the demand and high production cost associated with microalgal biomass make it necessary to implement novel strategie ...[more]

PMID: 31969931

Similar Datasets

Project description:Microbiota from rats fed with wheat aleurone and plant omega fatty acids In this study we investigated how an AX-rich WA and ALA from linseed oil (LO) modulate the gut microbiota of rats. Wistar rats were fed a standard diet and received either an iso-energetic control oil (PO), control oil + aleurone (A+PO), linseed oil (LO) or linseed oil + aleurone (A+LO) during 12 weeks. Feacal samples were recovered after the 12 week treatments. DNA extractions were performed using using the Qiagen's DNA Stool Kit (Qiagen, West Sussex, UK). 10ng of DNA template were amplified by PCR (16S gene) and purified using Qiagen's Qiaquick PCR purification kit (Qiagen, West Sussex, UK). 1ug of purified PCR product were labelled with either Cy3 or Cy5 using Genomic DNA ULS Labelling kit (Agilent Technologies, Palo Alto, CA). 250ng of labelled DNA were hybridized on the microarray for 24h at 65M-BM-0C. Washings were performed as recommended by the manufacturer. Microarray scanning was performed on a Surescan Microarray scanner (Agilent Technologies, Palo Alto, CA). Data were extracted using the Feature extraction software (Agilent Technologies, Palo Alto, CA). The retained intensity value for each probe was the ratio between the spotM-bM-^@M-^Ys median intensity signals and the median of background signals. A 13 chip study was realized to analyze the feacal microbiota of rats treated with either an iso-energetic control oil (PO), control oil + aleurone (A+PO), linseed oil (LO) or linseed oil + aleurone (A+LO) during 12 weeks. Each microarray corresponding to hybridization with 250ng of labelled 16S rRNA gene amplicons from 2 rat DNA faecal samples. Microbiota structure and diversity were assessed using the HuGChip (Tottey et al., 2013). Each probe (4441) was synthetized in three replicates. On the same array, 2 different samples were hybridized. One labelled with the Cy3 dye and one with the Cy5 dye. The results were processed as single channel (13 raw data files available on Series records for 25 samples).

Project description:Calorie restriction (CR) consistently extends longevity and delays age-related diseases across several animal models. We have previously shown that different dietary fat sources can modulate life span and mitochondrial ultrastructure, function and membrane fatty acid composition in mice maintained on a 40% CR. In particular, animals consuming lard as the main fat source (CR-Lard) lived longer than CR mice consuming diets with soybean oil (CR-Soy) or fish oil (CR-Fish) as the predominant lipid source. In the present work, a transcriptomic analysis in liver and skeletal muscle was performed in order to elucidate possible mechanisms underlying the changes in energy metabolism and longevity induced by dietary fat in CR mice. After 8 months of CR, transcription downstream of several mediators of inflammation was inhibited in liver. In contrast, proinflammatory signaling was increased in the CR-Fish versus other CR groups. Dietary fish oil induced a gene expression pattern consistent with increased transcriptional regulation by several cytokines (TNF, GM-CSF, TGF-b) and sex hormones when compared to the other CR groups. The CR-Fish also had lower expression of genes involved in fatty acid biosynthesis and increased expression of mitochondrial and peroxisomal fatty acid b-oxidation genes than the other CR diet groups. Our data suggest that a diet high in n-3 PUFA, partially reverts CR-related changes in gene expression of key processes, such as inflammation and steroid hormone signaling, and this may mitigate life span extension with CR in mice consuming diets high in fish oil. Sixteen male C57BL/6 mice were purchased at 14 weeks of age from the Jackson Laboratory (Sacramento, CA) and fed a commercial rodent chow diet (Harlan Teklad #7012). The mice were allowed ad libitum access to food and their food intake was monitored for two weeks. At 4 months of age, the animals were randomly assigned to four groups. The Control group received 95% of the daily ad libitum caloric intake (13.6 kcal/day) to avoid excessive weight gain and obesity, and the other three groups received 60% of the ad libitum consumption (8.6 kcal/day). A total of 31 mice samples (eight control, eight fish, eight lard and seven soy distributed in liver (n=16) and muscle (n=15) tissues) were analyzed by RNA-Sequencing. However, 2 samples (1071 control liver [JFM42H], 1125 fish muscle [JFM45D]) were discarded for further analysis after performing cluster analysis (they were clustering in a different groups). Also their RIN values were not as high as those of the other samples, indicating lower quality of the RNA.

Dataset Information

Plant hormone induced enrichment of Chlorella sp. omega-3 fatty acids.

Publications

Plant hormone induced enrichment of <i>Chlorella</i> sp. omega-3 fatty acids.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets