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Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate.

ABSTRACT: An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 20 batches of reaction. Compared with the commercial enzyme Lipozyme TLIM, half-life (t _1/2) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing.

SUBMITTER: Zheng J

PROVIDER: S-EPMC6969875 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate.

Zheng Jianyong J Wei Wei W Wang Shengfan S Li Xiaojun X Zhang Yinjun Y Wang Zhao Z

3 Biotech 20200118 2

An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 2 ...[more]

PMID: 32002342

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