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Genome-scale CRISPR screening for potential targets of ginsenoside compound K.

ABSTRACT: Ginsenosides exhibit a large variety of biological activities in maintaining physical health; however, the molecule underpinnings underlining these biological activities remain to be defined. Here, we took a cellular condition that compound K (CK) induces autophagic cell death in HeLa cells, and setup a high-throughput genetic screening using CRISPR technology. We have identified a number of CK-resistant and CK-sensitive genes, and further validated PMAIP1 as a CK-resistant gene and WASH1 as a CK-sensitive gene. Compound K treatment reduces the expression of WASH1, which further accelerates the autophagic cell death, highlighting WASH1 as an interesting downstream mediator of CK effects. Overall, our study offers an easy-to-adopt platform to study the functional mediators of ginsenosides, and provides a candidate list of genes that are potential targets of CK.

SUBMITTER: Yang Y

PROVIDER: S-EPMC6971025 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Genome-scale CRISPR screening for potential targets of ginsenoside compound K.

Yang Yuanyuan Y Liu Xiaojian X Li Shuang S Chen Yanhao Y Zhao Yongxu Y Wei Yuda Y Qiu Yan Y Liu Yan Y Zhou Zhihua Z Han Jun J Wu Guohao G Ding Qiurong Q

Cell death & disease 20200120 1

Ginsenosides exhibit a large variety of biological activities in maintaining physical health; however, the molecule underpinnings underlining these biological activities remain to be defined. Here, we took a cellular condition that compound K (CK) induces autophagic cell death in HeLa cells, and setup a high-throughput genetic screening using CRISPR technology. We have identified a number of CK-resistant and CK-sensitive genes, and further validated PMAIP1 as a CK-resistant gene and WASH1 as a C ...[more]

PMID: 31959745

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Dataset Information

Genome-scale CRISPR screening for potential targets of ginsenoside compound K.

Publications

Genome-scale CRISPR screening for potential targets of ginsenoside compound K.

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