A Screening Platform to Identify and Tailor Biocompatible Small-Molecule Catalysts.

ABSTRACT: Interfacing biocompatible, small-molecule catalysis with cellular metabolism promises a straightforward introduction of new function into organisms without the need for genetic manipulation. However, identifying and optimizing synthetic catalysts that perform new-to-nature transformations under conditions that support life is a cumbersome task. To enable the rapid discovery and fine-tuning of biocompatible catalysts, we describe a 96-well screening platform that couples the activity of synthetic catalysts to yield non-canonical amino acids from appropriate precursors with the subsequent incorporation of these nonstandard building blocks into GFP (quantifiable readout). Critically, this strategy does not only provide a common readout (fluorescence) for different reaction/catalyst combinatio

SUBMITTER: Rubini R 

PROVIDER: S-EPMC6972700 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature