Dataset Information

Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.

ABSTRACT: The enzyme activities of the fungus Lasiodiplodia theobromae (L. theobromae) were studied during degradation of benzo[a]pyrene (BaP). The L. theobromae was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil collected from the Beijing Coking Plant in China and can potentially use BaP as its sole carbon source with a degradation ratio of up to 53% over 10 days. The activities of lignin peroxidase (LiP) and laccase (LAC) could be detected during BaP biodegradation; while manganese peroxidase (MnP) was not detected. Both glucose and salicylic acid enhanced BaP biodegradation slightly. In contrast, the coexistence of phenanthrene (PHE) inhibited BaP degradation. These metabolic substrates all enhanced the secretion of LiP and LAC. The addition of Tween 80 (TW-80) enhanced BaP biodegradation as well as the LiP and LAC activities. At the same time, TW-80 was degraded by the L. theobromae. In addition, the L. theobromae was compared to Phanerochaete chrysosporium (P. chrysosporium), which is a widely studied fungus for degrading PAH. P. chrysosporium was unable to use BaP as its sole carbon source. The activities of LiP and LAC produced by the P. chrysosporium were less than those of the L. theobromae. Additionally, the four intermediates formed in the BaP biodegradation process were monitored using GC-MS analysis. Four metabolite concentrations first increased and then decreased or obtained the platform with prolonged BaP biodegradation time. Therefore, this study shows that the L. theobromae may be explored as a new strain for removing PAHs from the environment.

SUBMITTER: Cao H

PROVIDER: S-EPMC6972742 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.

Cao Huimin H Wang Cuiping C Liu Haibin H Jia Weili W Sun Hongwen H

Scientific reports 20200121 1

The enzyme activities of the fungus Lasiodiplodia theobromae (L. theobromae) were studied during degradation of benzo[a]pyrene (BaP). The L. theobromae was isolated from a polycyclic aromatic hydrocarbons (PAHs) contaminated soil collected from the Beijing Coking Plant in China and can potentially use BaP as its sole carbon source with a degradation ratio of up to 53% over 10 days. The activities of lignin peroxidase (LiP) and laccase (LAC) could be detected during BaP biodegradation; while mang ...[more]

PMID: 31964981

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Project description:Environmental alterations modulate host-microorganism interactions. Little is known about how climate changes can trigger pathogenic features on symbiont or mutualistic microorganisms. Current climate models predict increased environmental temperatures. The exposing of phytopathogens to these changing conditions can have particularly relevant consequences for economically important species and for humans. The impact on pathogen/host interaction and the shift on their biogeographical range can induce different levels of virulence in new hosts, allowing massive losses in agricultural and health fields. Lasiodiplodia theobromae is a phytopathogenic fungus responsible for a number of diseases in various plants. It has also been described as an opportunist pathogen in humans, causing infections with different levels of severity. L. theobromae has a high capacity of adaptation to different environments, such as woody plants, moist argillaceous soils, or even humans, being able to grow and infect hosts in a wide range of temperatures (9-39°C). Nonetheless, the effect of an increase of temperature, as predicted in climate change models, on L. theobromae is unknown. Here we explore the effect of temperature on two strains of L. theobromae - an environmental strain, CAA019, and a clinical strain, CBS339.90. We show that both strains are cytotoxic to mammalian cells but while the environmental strain is cytotoxic mainly at 25°C, the clinical strain is cytotoxic mainly at 30 and 37°C. Extracellular gelatinolytic, xylanolytic, amylolytic, and cellulolytic activities at 25 and 37°C were characterized by zymography and the secretome of both strains grown at 25, 30, and 37°C were characterized by electrophoresis and by Orbitrap LC-MS/MS. More than 75% of the proteins were identified, mostly enzymes (glycosyl hydrolases and proteases). The strains showed different protein profiles, which were affected by growth temperature. Also, strain specific proteins were identified, such as a putative f5/8 type c domain protein - known for being involved in pathogenesis - by strain CAA019 and a putative tripeptidyl-peptidase 1 protein, by strain CBS339.90. We showed that temperature modulates the secretome of L. theobromae. This modulation may be associated with host-specificity requirements. We show that the study of abiotic factors, such as temperature, is crucial to understand host/pathogen interactions and its impact on disease.

Project description:Understanding the potential for Polycyclic aromatic hydrocarbons (PAH) degradation by indigenous microbiota and the influence of PAHs on native microbial communities is of great importance for bioremediation and ecological evaluation. Various studies have focused on the bacterial communities in the environment where obvious PAH degradation was observed, little is known about the microbiota in the soil where poor degradation was observed. Soil microcosms were constructed with a red soil by supplementation with a high-molecular-weight PAH (pyrene) at three dosages (5, 30, and 70 mg ⋅ kg(-1)). Real-time PCR was used to evaluate the changes in bacterial abundance and pyrene dioxygenase gene (nidA) quantity. Illumina sequencing was used to investigate changes in diversity, structure, and composition of bacterial communities. After 42 days of incubation, no evident degradation was observed. The poor degradation ability was associated with the stability or significant decrease of abundance of the nidA gene. Although the abundance of the bacterial 16S rRNA gene was not affected by pyrene, the bacterial richness and diversity were decreased with increasing dosage of pyrene and the community structure was changed. Phylotypes affected by pyrene were comprehensively surveyed: (1) at the high taxonomic level, seven of the abundant phyla/classes (relative abundance >1.0%) including Chloroflexi, AD3, WPS-2, GAL5, Alphaproteobacteria, Actinobacteria, and Deltaproteobacteria and one rare phylum Crenarchaeota were significantly decreased by at least one dosage of pyrene, while three phyla/classes (Acidobacteria, Betaproteobacteria, and Gammaproteobacteria) were significantly increased; and (2) at the lower taxonomic level, the relative abundances of twelve orders were significantly depressed, whereas those of nine orders were significantly increased. This work enhanced our understanding of the biodegradation potential of pyrene in red soil and the effect of pyrene on soil ecosystems at the microbial community level.

Dataset Information

Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.

Publications

Enzyme activities during Benzo[a]pyrene degradation by the fungus Lasiodiplodia theobromae isolated from a polluted soil.

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