Considerations in adapting CRISPR/Cas9 in nongenetic model plant systems.

ABSTRACT: The past six years have seen the rapid growth of studies of CRISPR/Cas9 in plant genome editing, a method that enormously facilitates both basic research and practical applications. Most studies have focused on genetic model species, but plant species that are not genetic models may also be economically important or biologically significant, or both. However, developing the CRISPR/Cas9 system in a nongenetic model is challenging. Here, we summarize CRISPR/Cas9 applications in 45 plant genera across 24 families and provide a reference for practical application of CRISPR in nongenetic model plant systems. Suggestions for selecting plant species and target genes are given for proof-of-principle CRISPR studies, and the processes of vector construction are reviewed. We recommend using transient

SUBMITTER: Shan S 

PROVIDER: S-EPMC6976890 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature