Dataset Information

Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan.

ABSTRACT: To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal phenotypic mutants and many aberrant chlorophyll mutants at seedling stage. At mature stage, many semi-sterile mutants were observed. Dwarf, reduced culm number and lesion mimic mutants were also found. In total, 43.2% of the lines segregated some phenotypic mutants. Thus, the nDart1-tagged lines of Koshihikari are expected to be potentially useful for screening stress-tolerant mutants under abiotic or biotic stress conditions.

SUBMITTER: Nishimura H

PROVIDER: S-EPMC6977457 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan.

Nishimura Hideki H Himi Eiko E Rikiishi Kazuhide K Tsugane Kazuo K Maekawa Masahiko M

Breeding science 20191114 4

To utilize a transposon-tagged mutant as a breeding material in rice, an endogenous DNA transposon, nDart1-0, was introduced into Koshihikari by successive backcrossing together with aDart1-27, an active autonomous element. The founder line for nDart1-tagged lines of Koshihikari carried nDart1-0 on chromosome 9 and transposed nDart1-12s on chromosomes 1 and 8 and nDart1-3 on chromosome 11. In nDart1-tagged lines, there were the most abnormal pheno ...[more]

PMID: 31988635

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Establishment of nDart1-tagged lines of Koshihikari, an elite variety of rice in Japan.

Publications

Establishment of <i>nDart1</i>-tagged lines of Koshihikari, an elite variety of rice in Japan.

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