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Profiling of defense responsive pathway regulatory genes in Asian rice (Oryza sativa) against infection of Meloidogyne graminicola (Nematoda:Meloidogynidae).

ABSTRACT: In the present study, 36 Asian rice cultivars/landraces were evaluated against M. graminicola under in vitro conditions using soilless Pluronic gel medium. The cultivars/genotypes Phule Radha, EK 70, LK 248 and Khalibagh showed significantly reduced nematode infection, endoparasitic development, and derived multiplication factor indicating the presence of resistance, while Halvi Sal 17 was found to be most susceptible. Performance of selected genotypes showing resistance/susceptibility under in vitro conditions was further confirmed in soil which also revealed Phule Radha to be highly resistant and Halvi Sal 17 as the most susceptible genotype. Further, expression profile of plant defense responsive genes related to MAPK pathway, phytohormones, PR-proteins and callose and lignin synthesis were quantified in Phule Radha (the most resistant) and Halvi Sal 17 (the most susceptible) at 2 and 6 days post nematode inoculation. Significant upregulated expression of several defensive genes was observed in the resistant cultivar Phule Radha in contrast to insignificant expression in the susceptible varieties. The resistant genotype identified in the present study will be highly promising for resistance breeding in rice against M. graminicola.

SUBMITTER: Hatzade B

PROVIDER: S-EPMC6977811 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Profiling of defense responsive pathway regulatory genes in Asian rice (Oryza sativa) against infection of Meloidogyne graminicola (Nematoda:Meloidogynidae).

Hatzade Bhupal B Singh Divya D Phani Victor V Kumbhar Shailesh S Rao Uma U

3 Biotech 20200123 2

In the present study, 36 Asian rice cultivars/landraces were evaluated against M. graminicola under in vitro conditions using soilless Pluronic gel medium. The cultivars/genotypes Phule Radha, EK 70, LK 248 and Khalibagh showed significantly reduced nematode infection, endoparasitic development, and derived multiplication factor indicating the presence of resistance, while Halvi Sal 17 was found to be most susceptible. Performance of selected genotypes showing resistance/susceptibility un ...[more]

PMID: 32030329

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Project description:Meloidogyne polycephannulata n. sp. is described from specimens collected from an area cultivated with carrot cv. Brasilia, in the city of Rio Paranaíba, in the region of Alto Paranaíba, Minas Gerais State, Brazil. The perineal pattern of the female is circular to ovoid with a high dorsal arch that has widely spaced, coarse annulations. The lateral field may have a deep furrow separating the dorsal and ventral arches. The medial lips are short and wide, whereas the lateral lips are large and triangular. The female stylet is 15-16 ?m long with wide knobs, distinctly divided by an indentation in the center. Its tip is slightly curved dorsally. The excretory pore opens 34-65 ?m from the anterior end. Females retain eggs and second-stage juveniles in their body cavity, similar to that of the cyst-forming nematodes. Males are 1.3-1.7 mm long and have a high head cap that is rounded and slopes posteriorly. The labial disc is fused to the medial lips. The head region has several irregular annulations that are similar in appearance to the first or second body annules that are likewise irregular, making the head region appear to be extremely large. The stylet of the male is 21-24 ?m long; it is slender, and has small, rounded knobs, that are distinctly indented medially and appear heart-shaped. The shaft has several tiny projections throughout its length. Mean second-stage juvenile length is 411.7 ?m. The juvenile head cap is elevated, the medial lips are small, and the lateral lips are elongate to triangular-shaped. The head region has several short, incomplete and irregular transverse annulations. The juvenile stylet is 14-23 ?m long with small, rounded, and sloping knobs. The thin tail ends with a short hyaline portion that is variable in size (16-26 ?m) and with a small, rounded tip. Isozyme profiles of esterases from Meloidogyne javanica show 3 strong bands (SB) at Rm 46, 59, and 66; profiles of M. polycephannulata n. sp. show a SB at Rm 47 and a weak band (WB) at Rm 52; M. petuniae has two SB at Rm 44 and 53; M. phaseoli has a SB at 53, 58, and 64 Rm; M. brasilensis has three SB at Rm 40, 58, and 66 and a WB at Rm 71; M. pisi has a SB at Rm 40, 60, and 64 and two WB at 46 and 50 Rm. Data from sequencing the 18S rDNA region of M. polycephannulata n. sp. confirms that it is different from M. arabicida, M. arenaria, M. ethiopica, M. incognita, M. javanica, M. paranaensis, and M. thailandica. Sequence identity among these eight species ranged between 85 to 93.4%. Meloidogyne polycephannulata n. sp. reproduces very well on carrot and tomato; poorly on pepper; and not at all on cotton, peanut, tobacco, watermelon, and sweet corn.

Dataset Information

Profiling of defense responsive pathway regulatory genes in Asian rice (Oryza sativa) against infection of Meloidogyne graminicola (Nematoda:Meloidogynidae).

Publications

Profiling of defense responsive pathway regulatory genes in Asian rice (<i>Oryza sativa</i>) against infection of <i>Meloidogyne graminicola</i> (Nematoda:Meloidogynidae).

Similar Datasets

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