Hepatic ILC2 activity is regulated by liver inflammation-induced cytokines and effector CD4⁺ T cells.

ABSTRACT: In immune-mediated hepatitis, type 2 innate lymphoid cells (ILC2) as well as effector CD4⁺ T cells have been shown to drive disease pathology. However, less is known about mechanisms involved in the regulation of ILC2 function during liver inflammation. We showed that in homeostasis, hepatic ILC2 constituted a very small population with a naive, inactive phenotype. During immune-mediated hepatitis, the cytokines IL-33 and IFN? were expressed in liver tissue. IL-33 induced strong activation and expression of type 2 cytokines as well as IL-6 by hepatic ILC2 while IFN? suppressed cytokine production. Interestingly, this inhibitory effect was overcome by IL-33. The phenotype of activated hepatic ILC2 were stable since they did not show functional plasticity in response to liver inflammation-induced cytokines. Moreover, hepatic ILC2 induced a Th2 phenotype in activated CD4⁺ T cells, which increased ILC2-derived cytokine expression via IL-2. In contrast, Th1 cells inhibited survival of ILC2 by production of IFN?. Thus, hepatic ILC2 function is regulated by IL-33, IL-2, and IFN?. While IL-33 and IL-2 support hepatic ILC2 activation, their inflammatory activity in immune-mediated hepatitis might be limited by infiltrating IFN?-expressing Th1 cells.

SUBMITTER: Steinmann S 

PROVIDER: S-EPMC6978388 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature