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Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products.


ABSTRACT: Metabolomics is a powerful tool in the analysis and identification of metabolites responsible for biological properties. Regarding natural product chemistry, it constitutes a potential strategy to streamline the classic and laborious process of isolating natural products, which often involves the re-isolation and identification of known compounds. In this contribution, we establish a mass spectrometry-based metabolomics strategy to discover compounds with larvicidal activity against Aedes aegypti. We analyse the Brazilian plant Annona crassiflora using different platforms to annotate the active compounds in different extracts/fractions of various plant parts. The MetaboAnalyst and GNPS platforms, which consider LC-MS and LC-MS/MS data, respectively, were chosen to identify compounds that differentiate active and inactive samples. Bio-guided isolation was subsequently performed to confirm compound activity. Results proved the capacity of metabolomics to predict metabolite differences between active and inactive samples using LC-MS and LC-MS/MS data. Moreover, we discuss the limitations, possibilities, and strategies to have a broad view of vast data.

SUBMITTER: Demarque DP 

PROVIDER: S-EPMC6978511 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Mass spectrometry-based metabolomics approach in the isolation of bioactive natural products.

Demarque Daniel P DP   Dusi Renata G RG   de Sousa Francisco D M FDM   Grossi Sophia M SM   Silvério Maira R S MRS   Lopes Norberto P NP   Espindola Laila S LS  

Scientific reports 20200123 1


Metabolomics is a powerful tool in the analysis and identification of metabolites responsible for biological properties. Regarding natural product chemistry, it constitutes a potential strategy to streamline the classic and laborious process of isolating natural products, which often involves the re-isolation and identification of known compounds. In this contribution, we establish a mass spectrometry-based metabolomics strategy to discover compounds with larvicidal activity against Aedes aegypt  ...[more]

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