Stability and profiling of urinary microRNAs in healthy cats and cats with pyelonephritis or other urological conditions.

ABSTRACT:

Background

Specific biomarkers of pyelonephritis (PN) in cats are lacking. MicroRNAs (miRNAs) have diagnostic potential in human nephropathies.

Objectives

To investigate the presence/stability of miRNAs in whole urine of cats and the discriminatory potential of selected urinary miRNAs for PN in cats.

Animals

Twelve healthy cats, 5 cats with PN, and 13 cats with chronic kidney disease (n = 5), subclinical bacteriuria (n = 3), and ureteral obstructions (n = 5) recruited from 2 companion animal hospitals.

Methods

Prospective case-control study. Expression profiles of 24 miRNAs were performed by quantitative PCR (qPCR). Effect of storage temperature (4°C [24?hours], -20°C, and -80°C) was determined for a subset of miRNAs in healthy cats.

Results

Urinary miR-4286, miR-30c, miR-204, miR4454, miR-21, miR-16, miR-191, and miR-30a were detected. For the majority of miRNAs tested, storage at 4°C and -20°C resulted in significantly lower miRNA yield compared to storage at -80°C (mean log2fold changes across miRNAs from -0.5 ?±?0.4 SD to -1.20?±?0.4 SD (4°C versus -80°C) and from -0.7?±?0.2 SD to -1.20?±?0.3 SD (-20°C versus -80°C)). Cats with PN had significantly upregulated miR-16 with a mean log2fold change of 1.0?±?0.4 SD, compared with controls (-0.1?±?0.2, P = .01) and other urological conditions (0.6?±?0.3, P = .04).

Conclusions

Upregulation of miR16 might be PN-specific, pathogen-specific (Escherichia coli), or both.

SUBMITTER: Jessen LR 

PROVIDER: S-EPMC6979273 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature