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HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.


ABSTRACT: World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94) with a mean of differences of 0.23 log10 copies/mL. Using the 1,000 copies/mL cut-off, the sensitivity of DBS was 97% (95%CI, 91-100%) and specificity was 75% (95%CI, 19-99%). DBS are useful to scale-up HIV RNA VL testing in settings with limited access to VL testing.

SUBMITTER: Khamduang W 

PROVIDER: S-EPMC6980635 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.

Khamduang Woottichai W   Kaewbundit Ampika A   Duangmano Amonrat A   Hongjaisee Sayamon S   Klinbuayaem Virat V   Halue Guttiga G   Chutanunta Apichat A   Sirirungsi Wasna W   Jourdain Gonzague G   Ngo-Giang-Huong Nicole N  

PloS one 20200124 1


World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals of two different regions of Thailand were shipped to a central laboratory along with corresponding plasma specimens. Viral load was measured in both DBS and plasma using the Abbott m2000 system. HIV RNA levels were strongly correlated (r = 0.94)  ...[more]

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