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Detecting Counterion Dynamics in DNA-Protein Association.

ABSTRACT: Due to a high density of negative charges on its surface, DNA condenses cations as counterions, forming the so-called "ion atmosphere". Although the release of counterions upon DNA-protein association has been postulated to have a major contribution to the binding thermodynamics, this release remains to be confirmed through a direct observation of the ions. Herein, we report the characterization of the ion atmosphere around DNA using NMR spectroscopy and directly detect the release of counterions upon DNA-protein association. NMR-based diffusion data reveal the highly dynamic nature of counterions within the ion atmosphere around DNA. Counterion release is observed as an increase in the apparent ionic diffusion coefficient, which directly provides the number of counterions released upon DNA-protein association.

SUBMITTER: Pletka CC

PROVIDER: S-EPMC6980997 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Detecting Counterion Dynamics in DNA-Protein Association.

Pletka Channing C CC Nepravishta Ridvan R Iwahara Junji J

Angewandte Chemie (International ed. in English) 20191203 4

Due to a high density of negative charges on its surface, DNA condenses cations as counterions, forming the so-called "ion atmosphere". Although the release of counterions upon DNA-protein association has been postulated to have a major contribution to the binding thermodynamics, this release remains to be confirmed through a direct observation of the ions. Herein, we report the characterization of the ion atmosphere around DNA using NMR spectroscopy and directly detect the release of counterion ...[more]

PMID: 31743557

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