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Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins.

ABSTRACT: Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a model multi-pass membrane protein, we demonstrate how incorporation of a multi-pass membrane protein into nanodiscs can be used in flow cytometry to identify antigen-specific hybridoma. The use of target protein-loaded nanodiscs to sort individual hybridoma early in the screening process can reduce the time required to identify antibodies against multi-pass membrane proteins.

SUBMITTER: Gardill B

PROVIDER: S-EPMC6981118 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Nanodisc technology facilitates identification of monoclonal antibodies targeting multi-pass membrane proteins.

Gardill Bernd B Huang Jerry J Tu Lawrence L Van Petegem Filip F Oxenoid Kirill K Thomson Christy A CA

Scientific reports 20200124 1

Multi-pass membrane proteins are important targets of biologic medicines. Given the inherent difficulties in working with membrane proteins, we sought to investigate the utility of membrane scaffold protein nanodiscs as a means of solubilizing membrane proteins to aid antibody discovery. Using a model multi-pass membrane protein, we demonstrate how incorporation of a multi-pass membrane protein into nanodiscs can be used in flow cytometry to identify antigen-specific hybridoma. The use of target ...[more]

PMID: 31980674

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