Ontology highlight
ABSTRACT:
SUBMITTER: Li K
PROVIDER: S-EPMC6981169 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Li Kailong K Liu Yuxuan Y Cao Hui H Zhang Yuannyu Y Gu Zhimin Z Liu Xin X Yu Andy A Kaphle Pranita P Dickerson Kathryn E KE Ni Min M Xu Jian J
Nature communications 20200124 1
Tissue-specific gene expression requires coordinated control of gene-proximal and -distal cis-regulatory elements (CREs), yet functional analysis of gene-distal CREs such as enhancers remains challenging. Here we describe CRISPR/dCas9-based enhancer-targeting epigenetic editing systems, enCRISPRa and enCRISPRi, for efficient analysis of enhancer function in situ and in vivo. Using dual effectors capable of re-writing enhancer-associated chromatin modifications, we show that enCRISPRa and enCRISP ...[more]