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Live imaging of neurogenesis in the adult mouse hippocampus.

ABSTRACT: Neural stem and progenitor cells (NSPCs) generate neurons throughout life in the mammalian hippocampus. We used chronic in vivo imaging and followed genetically labeled individual NSPCs and their progeny in the mouse hippocampus for up to 2 months. We show that NSPCs targeted by the endogenous Achaete-scute homolog 1 (Ascl1) promoter undergo limited rounds of symmetric and asymmetric divisions, eliciting a burst of neurogenic activity, after which they are lost. Further, our data reveal unexpected asymmetric divisions of nonradial glia-like NSPCs. Cell fates of Ascl1-labeled lineages suggest a developmental-like program involving a sequential transition from a proliferative to a neurogenic phase. By providing a comprehensive description of lineage relationships, from dividing NSPCs to newborn neurons integrating into the hippocampal circuitry, our data offer insight into how NSPCs support life-long hippocampal neurogenesis.

SUBMITTER: Pilz GA

PROVIDER: S-EPMC6986926 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Live imaging of neurogenesis in the adult mouse hippocampus.

Pilz Gregor-Alexander GA Bottes Sara S Betizeau Marion M Jörg David J DJ Carta Stefano S April Simon S Simons Benjamin D BD Helmchen Fritjof F Jessberger Sebastian S

Science (New York, N.Y.) 20180201 6376

Neural stem and progenitor cells (NSPCs) generate neurons throughout life in the mammalian hippocampus. We used chronic in vivo imaging and followed genetically labeled individual NSPCs and their progeny in the mouse hippocampus for up to 2 months. We show that NSPCs targeted by the endogenous Achaete-scute homolog 1 (Ascl1) promoter undergo limited rounds of symmetric and asymmetric divisions, eliciting a burst of neurogenic activity, after which they are lost. Further, our data reveal unexpect ...[more]

PMID: 29439238

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