Ontology highlight
ABSTRACT:
SUBMITTER: Arora S
PROVIDER: S-EPMC6988089 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Arora Simran S Horne W Seth WS Islam Kabirul K
Journal of the American Chemical Society 20190920 39
Protein-protein interactions mediated by methyllysine are ubiquitous in biological systems. Specific perturbation of such interactions has remained a challenging endeavor. Herein, we describe an allele-specific strategy toward an engineered protein-protein interface orthogonal to the human proteome. We develop a methyltransferase (writer) variant that installs aryllysine moiety on histones that can only be recognized by an engineered chromodomain (reader). We establish biochemical integrity of t ...[more]