Dataset Information

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803.

ABSTRACT:

Background

Cyanobacteria can be metabolically engineered to convert CO₂ to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules.

Results

The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed significantly increased ethylene production (10.5 ± 3.1 µg mL^-1 OD^-1 day^-1) compared to the control strain (6.4 ± 1.4 µg mL^-1 OD^-1 day^-1). Interestingly, extra copies of the native pepc or the heterologous expression of PEPc from the cyanobacterium Synechococcus PCC 7002 (Synechococcus) in the CD-P, increased ethylene production (19.2 ± 1.3 and 18.3 ± 3.3 µg mL^-1 OD^-1 day^-1, respectively) when the cells were treated with the acetyl-CoA carboxylase inhibitor, cycloxydim. A heterologous expression of phosphoenolpyruvate synthase (PPSA) from Synechococcus in the CD-P also increased ethylene production (16.77 ± 4.48 µg mL^-1 OD^-1 day^-1) showing differences in the regulation of the native and the PPSA from Synechococcus in Synechocystis.

Conclusions

This work demonstrates that genetic rewiring of cyanobacterial central carbon metabolism can enhance carbon supply to the TCA cycle and thereby further increase ethylene production.

SUBMITTER: Durall C

PROVIDER: S-EPMC6988332 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Publications

Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium <i>Synechocystis</i> PCC 6803.

Durall Claudia C Lindberg Pia P Yu Jianping J Lindblad Peter P

Biotechnology for biofuels 20200128

<h4>Background</h4>Cyanobacteria can be metabolically engineered to convert CO<sub>2</sub> to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules.<h4>Results</h4>The <i>efe</i> gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium <i>Synechocystis</i> PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed sig ...[more]

PMID: 32010220

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