Localization microscopy at doubled precision with patterned illumination.
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ABSTRACT:
SUBMITTER: Cnossen J
PROVIDER: S-EPMC6989044 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Publications
Localization microscopy at doubled precision with patterned illumination.
Cnossen Jelmer J Hinsdale Taylor T Thorsen Rasmus Ø RØ Siemons Marijn M Schueder Florian F Jungmann Ralf R Smith Carlas S CS Rieger Bernd B Stallinga Sjoerd S
Nature methods 20191209 1
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM ...[more]
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