Localization microscopy at doubled precision with patterned illumination.

ABSTRACT: MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.

SUBMITTER: Cnossen J 

PROVIDER: S-EPMC6989044 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature