Ontology highlight
ABSTRACT:
SUBMITTER: Celaj A
PROVIDER: S-EPMC6989212 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Celaj Albi A Gebbia Marinella M Musa Louai L Cote Atina G AG Snider Jamie J Wong Victoria V Ko Minjeong M Fong Tiffany T Bansal Paul P Mellor Joseph C JC Seesankar Gireesh G Nguyen Maria M Zhou Shijie S Wang Liangxi L Kishore Nishka N Stagljar Igor I Suzuki Yo Y Yachie Nozomu N Roth Frederick P FP
Cell systems 20191023 1
Many traits are complex, depending non-additively on variant combinations. Even in model systems, such as the yeast S. cerevisiae, carrying out the high-order variant-combination testing needed to dissect complex traits remains a daunting challenge. Here, we describe "X-gene" genetic analysis (XGA), a strategy for engineering and profiling highly combinatorial gene perturbations. We demonstrate XGA on yeast ABC transporters by engineering 5,353 strains, each deleted for a random subset of 16 tra ...[more]