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Microchannel network hydrogel induced ischemic blood perfusion connection.

ABSTRACT: Angiogenesis induction into damaged sites has long been an unresolved issue. Local treatment with pro-angiogenic molecules has been the most common approach. However, this approach has critical side effects including inflammatory coupling, tumorous vascular activation, and off-target circulation. Here, the concept that a structure can guide desirable biological function is applied to physically engineer three-dimensional channel networks in implant sites, without any therapeutic treatment. Microchannel networks are generated in a gelatin hydrogel to overcome the diffusion limit of nutrients and oxygen three-dimensionally. Hydrogel implantation in mouse and porcine models of hindlimb ischemia rescues severely damaged tissues by the ingrowth of neighboring host vessels with microchannel perfusion. This effect is guided by microchannel size-specific regenerative macrophage polarization with the consequent functional recovery of endothelial cells. Multiple-site implantation reveals hypoxia and neighboring vessels as major causative factors of the beneficial function. This technique may contribute to the development of therapeutics for hypoxia/inflammatory-related diseases.

SUBMITTER: Lee JB

PROVIDER: S-EPMC6992688 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Microchannel network hydrogel induced ischemic blood perfusion connection.

Lee Jung Bok JB Kim Dae-Hyun DH Yoon Jeong-Kee JK Park Dan Bi DB Kim Hye-Seon HS Shin Young Min YM Baek Wooyeol W Kang Mi-Lan ML Kim Hyun Jung HJ Sung Hak-Joon HJ

Nature communications 20200130 1

Angiogenesis induction into damaged sites has long been an unresolved issue. Local treatment with pro-angiogenic molecules has been the most common approach. However, this approach has critical side effects including inflammatory coupling, tumorous vascular activation, and off-target circulation. Here, the concept that a structure can guide desirable biological function is applied to physically engineer three-dimensional channel networks in implant sites, without any therapeutic treatment. Micro ...[more]

PMID: 32001693

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Project description:PurposeTo perform a proof-of-concept study to investigate the clinical utility of perfusion maps derived from convolutional neural networks (CNNs) for the workup of patients with acute ischemic stroke presenting with a large vessel occlusion.Materials and methodsData on endovascularly treated patients with acute ischemic stroke (n = 151; median age, 68 years [interquartile range, 59-75 years]; 82 of 151 [54.3%] women) were retrospectively extracted from a single-center institutional prospective registry (between January 2011 and December 2015). Dynamic susceptibility perfusion imaging data were processed by applying a commercially available reference method and in parallel by a recently proposed CNN method to automatically infer time to maximum of the tissue residue function (Tmax) perfusion maps. The outputs were compared by using quantitative markers of tissue at risk derived from manual segmentations of perfusion lesions from two expert raters.ResultsStrong correlations of lesion volumes (Tmax > 4 seconds, > 6 seconds, and > 8 seconds; R = 0.865-0.914; P < .001) and good spatial overlap of respective lesion segmentations (Dice coefficients, 0.70-0.85) between the CNN method and reference output were observed. Eligibility for late-window reperfusion treatment was feasible with use of the CNN method, with complete interrater agreement for the CNN method (Cohen κ = 1; P < .001), although slight discrepancies compared with the reference-based output were observed (Cohen κ = 0.609-0.64; P < .001). The CNN method tended to underestimate smaller lesion volumes, leading to a disagreement between the CNN and reference method in five of 45 patients (9%).ConclusionCompared with standard deconvolution-based processing of raw perfusion data, automatic CNN-derived Tmax perfusion maps can be applied to patients who have acute ischemic large vessel occlusion stroke, with similar clinical utility.© RSNA, 2019Supplemental material is available for this article.

Dataset Information

Microchannel network hydrogel induced ischemic blood perfusion connection.

Publications

Microchannel network hydrogel induced ischemic blood perfusion connection.

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