Ontology highlight
ABSTRACT:
SUBMITTER: Krawczyk K
PROVIDER: S-EPMC6992713 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Krawczyk Krzysztof K Scheller Leo L Kim Hyojin H Fussenegger Martin M
Nature communications 20200130 1
Rewiring cellular sensors to trigger non-natural responses is fundamental for therapeutic cell engineering. Current designs rely on engineered receptors that are limited to single inputs, and often suffer from high leakiness and low fold induction. Here, we present Generalized Engineered Activation Regulators (GEARs) that overcome these limitations by being pathway-specific rather than input-specific. GEARs consist of the MS2 bacteriophage coat protein fused to regulatory or transactivation doma ...[more]