Ontology highlight
ABSTRACT:
SUBMITTER: Sheinberger J
PROVIDER: S-EPMC6994078 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Sheinberger Jonathan J Hochberg Hodaya H Lavi Erez E Kanter Itamar I Avivi Shira S Reinitz Gita G Schwed Avital A Aizler Yuval Y Varon Eli E Kinor Noa N Shav-Tal Yaron Y
Biology methods & protocols 20170101 1
Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (CD-tagging), which is based on a tag insertion into an endogenous gene by creation of a new exon. Previously, CD-tagging was used to tag endogenous proteins. Here we developed a CD-tagging-MS2 approach in which two tags were inserted in tandem; a fluorescent pr ...[more]