Ontology highlight
ABSTRACT:
SUBMITTER: Wise EL
PROVIDER: S-EPMC6994106 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
PLoS neglected tropical diseases 20200121 1
Oropouche virus (OROV) is responsible for outbreaks of Oropouche fever in parts of South America. We recently identified and isolated OROV from a febrile Ecuadorian patient, however, a previously published qRT-PCR assay did not detect OROV in the patient sample. A primer mismatch to the Ecuadorian OROV lineage was identified from metagenomic sequencing data. We report the optimisation of an qRT-PCR assay for the Ecuadorian OROV lineage, which subsequently identified a further five cases in a coh ...[more]