Ontology highlight
ABSTRACT:
SUBMITTER: Song H
PROVIDER: S-EPMC6994237 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Song Hongki H Orr Amy S AS Lee Miriam M Harner Max E ME Wickner William T WT
eLife 20200121
Yeast vacuole fusion requires R-SNARE, Q-SNAREs, and HOPS. A HOPS SM-family subunit binds the R- and Qa-SNAREs. We now report that HOPS binds each of the four SNAREs. HOPS catalyzes fusion when the Q-SNAREs are not pre-assembled, ushering them into a functional complex. Co-incubation of HOPS, proteoliposomes bearing R-SNARE, and proteoliposomes with any two Q-SNAREs yields a rapid-fusion complex with 3 SNAREs in a <i>trans</i>-assembly. The missing Q-SNARE then induces sudden fusion. HOPS can 't ...[more]