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Identification of functional regulatory elements in the human genome using pooled CRISPR screens.

ABSTRACT: BACKGROUND:Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function. However, protein-coding genes comprise

SUBMITTER: Borys SM

PROVIDER: S-EPMC6995077 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Identification of functional regulatory elements in the human genome using pooled CRISPR screens.

Borys Samantha M SM Younger Scott T ST

BMC genomics 20200131 1

<h4>Background</h4>Genome-scale pooled CRISPR screens are powerful tools for identifying genetic dependencies across varied cellular processes. The vast majority of CRISPR screens reported to date have focused exclusively on the perturbation of protein-coding gene function. However, protein-coding genes comprise < 2% of the sequence space in the human genome leaving a substantial portion of the genome uninterrogated. Noncoding regions of the genome harbor important regulatory elements (e.g. prom ...[more]

PMID: 32005150

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