Ontology highlight
ABSTRACT:
SUBMITTER: Zhou J
PROVIDER: S-EPMC6995172 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Zhou Junhua J Zhao Minqiong M Sun Zefang Z Wu Feilong F Liu Yucong Y Liu Xianghua X He Zuping Z He Quanze Q He Quanyuan Q
BMC bioinformatics 20200131 1
<h4>Background</h4>Whole genome bisulfite sequencing (WGBS) also known as BS-seq has been widely used to measure the methylation of whole genome at single-base resolution. One of the key steps in the assay is converting unmethylated cytosines into thymines (BS conversion). Incomplete conversion of unmethylated cytosines can introduce false positive methylation call. Developing a quick method to evaluate bisulfite conversion ratio (BCR) is benefit for both quality control and data analysis of WGB ...[more]