Ontology highlight
ABSTRACT:
SUBMITTER: Elling U
PROVIDER: S-EPMC6997032 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Nature protocols 20190603 7
Ploidy represents the number of chromosome sets in a cell. Although gametes have a haploid genome (n), most mammalian cells have diploid genomes (2n). The diploid status of most cells correlates with the number of probable alleles for each autosomal gene and makes it difficult to target these genes via mutagenesis techniques. Here, we describe a 7-week protocol for the derivation of mouse haploid embryonic stem cells (hESCs) from female gametes that also outlines how to maintain the cells once d ...[more]