Ontology highlight
ABSTRACT:
SUBMITTER: Zhong Z
PROVIDER: S-EPMC6997322 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Zhong Zhaohui Z Liu Shishi S Liu Xiaopei X Liu Binglin B Tang Xu X Ren Qiurong Q Zhou Jianping J Zheng Xuelian X Qi Yiping Y Zhang Yong Y
Rice (New York, N.Y.) 20200203 1
<h4>Background</h4>Expression of either Cas9 or Cas12a and guide RNAs by a single Polymerase II (Pol II) promoter represents a compact CRISPR expression system and has many advantages for different applications. In order to make this system routine in plant biology, engineering efforts are needed for developing and optimizing such single transcript unit (STU) systems for plant genome editing.<h4>Results</h4>To develop novel intron-based STU (iSTU) CRISPR system (STU CRISPR 3.0), we first evaluat ...[more]