Dataset Information

Olive fruit volatiles route intraspecific interactions and chemotaxis in Bactrocera oleae (Rossi) (Diptera: Tephritidae) females.

ABSTRACT: Plant nutritional quality and chemical characteristics may affect the fitness of phytophagous insects. Here, the olfactory preferences of Bactrocera oleae (Rossi) females toward olives with different maturation and infestation status were evaluated in three cultivars: Ottobratica, Roggianella and Sinopolese. Volatile profiles from olives were identified by SPME/GC-MS. Choice tests were performed to determine the responses of B. oleae adult females toward fruits and pure chemicals linked to infestation degree. Cultivar was the main source of variability explaining the differences recorded in volatile emissions. Moreover, three VOCs [?-myrcene, limonene and (E)-?-ocimene] were associated to infestation status across all olive varieties. In choice-tests, B. oleae females always preferred the olfactory cues from low-infested over high-infested fruits. Therefore, choice-tests using synthetic VOCs, emitted in greater amount by high-infested fruit, were arranged in order to identify putative B. oleae kairomones. While females were indifferent to ?-myrcene, the highest dosages of limonene and (E)-?-ocimene were unfavoured by the tested flies, which preferentially moved toward the empty arm of the Y-tube. Furthermore, females preferred the lowest concentration of ?-ocimene compared to the highest one. These results supported our hypothesis that fruit VOCs may serve as kairomones for female flies.

SUBMITTER: Giunti G

PROVIDER: S-EPMC6997409 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Olive fruit volatiles route intraspecific interactions and chemotaxis in Bactrocera oleae (Rossi) (Diptera: Tephritidae) females.

Giunti Giulia G Campolo Orlando O Laudani Francesca F Algeri Giuseppe Massimo GM Palmeri Vincenzo V

Scientific reports 20200203 1

Plant nutritional quality and chemical characteristics may affect the fitness of phytophagous insects. Here, the olfactory preferences of Bactrocera oleae (Rossi) females toward olives with different maturation and infestation status were evaluated in three cultivars: Ottobratica, Roggianella and Sinopolese. Volatile profiles from olives were identified by SPME/GC-MS. Choice tests were performed to determine the responses of B. oleae adult females toward fruits and pure chemicals linked to infes ...[more]

PMID: 32015351

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Project description:BACKGROUND: The Tephritidae family of insects includes the most important agricultural pests of fruits and vegetables, belonging mainly to four genera (Bactrocera, Ceratitis, Anastrepha and Rhagoletis). The olive fruit fly, Bactrocera oleae, is the major pest of the olive fruit. Currently, its control is based on chemical insecticides. Environmentally friendlier methods have been attempted in the past (Sterile Insect Technique), albeit with limited success. This was mainly attributed to the lack of knowledge on the insect's behaviour, ecology and genetic structure of natural populations. The development of molecular markers could facilitate the access in the genome and contribute to the solution of the aforementioned problems. We chose to focus on microsatellite markers due to their abundance in the genome, high degree of polymorphism and easiness of isolation. RESULTS: Fifty-eight microsatellite-containing clones were isolated from the olive fly, Bactrocera oleae, bearing a total of sixty-two discrete microsatellite motifs. Forty-two primer pairs were designed on the unique sequences flanking the microsatellite motif and thirty-one of them amplified a PCR product of the expected size. The level of polymorphism was evaluated against wild and laboratory flies and the majority of the markers (93.5%) proved highly polymorphic. Thirteen of them presented a unique position on the olive fly polytene chromosomes by in situ hybridization, which can serve as anchors to correlate future genetic and cytological maps of the species, as well as entry points to the genome. Cross-species amplification of these markers to eleven Tephritidae species and sequencing of thirty-one of the amplified products revealed a varying degree of conservation that declines outside the Bactrocera genus. CONCLUSION: Microsatellite markers are very powerful tools for genetic and population analyses, particularly in species deprived of any other means of genetic analysis. The presented set of microsatellite markers possesses all features that would render them useful in such analyses. This could also prove helpful for species where SIT is a desired outcome, since the development of effective SIT can be aided by detailed knowledge at the genetic and molecular level. Furthermore, their presented efficacy in several other species of the Tephritidae family not only makes them useful for their analysis but also provides tools for phylogenetic comparisons among them.

Dataset Information

Olive fruit volatiles route intraspecific interactions and chemotaxis in Bactrocera oleae (Rossi) (Diptera: Tephritidae) females.

Publications

Olive fruit volatiles route intraspecific interactions and chemotaxis in Bactrocera oleae (Rossi) (Diptera: Tephritidae) females.

Similar Datasets

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