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Comparative analysis of endophytic bacterial diversity between two varieties of sunflower Helianthus annuus with their PGP evaluation.

ABSTRACT: Endophytic bacterial diversity shows an intricate network of interactions with host plants as they reside in various tissues and organs at certain stages or all stages of their life cycle stimulating the plant growth and fitness. Sunflower is a trendy oilfield crop and variation in its varieties is associated with the dynamics of endophytic diversity. The present study is undertaken to identify and compare the ecological niche of endophytic bacterial communities amongst different tissues of two hybrids varieties Hysun-33 and Hysun-39 of sunflower (Helianthus annuus) at three developmental stages which are vegetative stage I (after 15 days of seeds germination), vegetative stage II (after 30 days of germination) and reproductive stage (after 90 days of germination). A total of 74 endophytes from Hysun-33 and 115 endophytes from Hysun-39 have been isolated from different tissues and growth stages. Amongst plant parts, root tissues harbored higher bacterial inhabitants (44) followed by stem (33), leaf (30) and flower (7) of Hysun-39. Likewise, Hysun-33 endophytes colonized roots more abundantly followed by leaves, stem and flowers. All strains are found to be gram positive with the exception of only RA9 from Hysun-33 and RB9 from Hysun-39 that are gram negative. Among different growth stages, the maximum bacterial population (CFU of 320 × 103) was found amongst root microflora at vegetative stage II of plant in Hysun-39 variety as compared to root endophytes of Hysun-33 having (CFU of 10 × 103). The evaluation of their growth promoting features revealed that among 74 isolates of Hysun-33, 70% exhibited the ability of hydrogen cyanide production, 43% IAA production, 36% siderophore production and 4% nitrogen fixation and also phosphate solubilization. However among 115 isolates of Hysun-39, 64% appeared as hydrogen cyanide producers, 56% IAA producers, 33% siderophore producers, 2% nitrogen fixers and 4% as phosphate solubilizers. Therefore our study reveals understanding of wide-ranging diversity of endophytic bacteria and their beneficial relationship with internal tissues of host plant which may recommend their implementation to crops for better development of agricultural systems.

SUBMITTER: Bashir S

PROVIDER: S-EPMC6997864 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Comparative analysis of endophytic bacterial diversity between two varieties of sunflower <i>Helianthus annuus</i> with their PGP evaluation.

Bashir Sadia S Iqbal Atia A Hasnain Shahida S

Saudi journal of biological sciences 20191217 2

Endophytic bacterial diversity shows an intricate network of interactions with host plants as they reside in various tissues and organs at certain stages or all stages of their life cycle stimulating the plant growth and fitness. Sunflower is a trendy oilfield crop and variation in its varieties is associated with the dynamics of endophytic diversity. The present study is undertaken to identify and compare the ecological niche of endophytic bacterial communities amongst different tissues of two ...[more]

PMID: 32210694

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Project description:Background: Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools. Methodology: Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensionally separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoprotein allergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry (MALDI TOF/TOF and LC ESI qTOF). MASCOT searching was performed against NCBInr database. However, Helianthus annuus genome is not fully sequenced and partially annotated. So in case of low confidence (p> 0.05) protein identification, searching was performed against EST library of Helianthus annuus. Results: Prevalence of sunflower pollen allergy was observed among 21% of the atopic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient serum detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two non-isoformic pectate lyases and a cystein protease. Conclusion: Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.

Dataset Information

Comparative analysis of endophytic bacterial diversity between two varieties of sunflower Helianthus annuus with their PGP evaluation.

Publications

Comparative analysis of endophytic bacterial diversity between two varieties of sunflower <i>Helianthus annuus</i> with their PGP evaluation.

Similar Datasets

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