Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration.
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ABSTRACT:
SUBMITTER: Tosstorff A
PROVIDER: S-EPMC6999263 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
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Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration.
Tosstorff Andreas A Kroner Cora C Opperman Diederik J DJ Hollmann Frank F Holtmann Dirk D
Engineering in life sciences 20161012 1
Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were iden ...[more]
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