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Anaerobic digestion of chicken manure: Influence of trace element supplementation.

ABSTRACT: In this study, anaerobic digestion of nitrogen-rich chicken (egg-laying hen) manure at different trace element (TE) mix doses and different total ammonia nitrogen (TAN) concentrations was investigated in batch digestion experiments. With respect to nonsupplemented TE sets, addition of TE mixture containing 1 mg/L Ni, 1 mg/L Co, 0.2 mg/L Mo, 0.2 mg/L Se, 0.2 mg/L W, and 5 mg/L Fe at TAN concentrations of 3000 mg/L and 4000 mg/L, cumulative CH4 production and CH4 production rate improved by 7-8% and 5-6%, respectively. The results revealed that at a very high TAN concentration of 6000 mg/L, the effect of TE addition was significantly high and the cumulative CH4 production and production rate were increased by 20 and 39.5%, respectively. Therefore, it is concluded that at elevated TAN concentrations the CH4 production that was stimulated by TE supplementation was presumably occurred through syntrophic acetate oxidation.

SUBMITTER: Molaey R

PROVIDER: S-EPMC6999301 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Anaerobic digestion of chicken manure: Influence of trace element supplementation.

Molaey Rahim R Bayrakdar Alper A Sürmeli Recep Önder RÖ Çalli Bariş B

Engineering in life sciences 20181220 2

In this study, anaerobic digestion of nitrogen-rich chicken (egg-laying hen) manure at different trace element (TE) mix doses and different total ammonia nitrogen (TAN) concentrations was investigated in batch digestion experiments. With respect to nonsupplemented TE sets, addition of TE mixture containing 1 mg/L Ni, 1 mg/L Co, 0.2 mg/L Mo, 0.2 mg/L Se, 0.2 mg/L W, and 5 mg/L Fe at TAN concentrations of 3000 mg/L and 4000 mg/L, cumulative CH<sub>4</sub> production and CH<sub>4</sub> production r ...[more]

PMID: 32624996

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Project description:Trace elements (TE) play an essential role in all organisms due to their functions in enzyme complexes. In anaerobic digesters, control, and supplementation of TEs lead to stable and more efficient methane production processes while TE deficits cause process imbalances. However, the underlying metabolic mechanisms and the adaptation of the affected microbial communities to such deficits are not yet fully understood. Here, we investigated the microbial community dynamics and resulting process changes induced by TE deprivation. Two identical lab-scale continuous stirred tank reactors fed with distiller's grains and supplemented with TEs (cobalt, molybdenum, nickel, tungsten) and a commercial iron additive were operated in parallel. After 72 weeks of identical operation, the feeding regime of one reactor was changed by omitting TE supplements and reducing the amount of iron additive. Both reactors were operated for further 21 weeks. Various process parameters (biogas production and composition, total solids and volatile solids, TE concentration, volatile fatty acids, total ammonium nitrogen, total organic acids/alkalinity ratio, and pH) and the composition and activity of the microbial communities were monitored over the total experimental time. While the methane yield remained stable, the concentrations of hydrogen sulfide, total ammonia nitrogen, and acetate increased in the TE-depleted reactor compared to the well-supplied control reactor. Methanosarcina and Methanoculleus dominated the methanogenic communities in both reactors. However, the activity ratio of these two genera was shown to depend on TE supplementation explainable by different TE requirements of their energy conservation systems. Methanosarcina dominated the well-supplied anaerobic digester, pointing to acetoclastic methanogenesis as the dominant methanogenic pathway. Under TE deprivation, Methanoculleus and thus hydrogenotrophic methanogenesis was favored although Methanosarcina was not overgrown by Methanoculleus. Multivariate statistics revealed that the decline of nickel, cobalt, molybdenum, tungsten, and manganese most strongly influenced the balance of mcrA transcripts from both genera. Hydrogenotrophic methanogens seem to be favored under nickel- and cobalt-deficient conditions as their metabolism requires less nickel-dependent enzymes and corrinoid cofactors than the acetoclastic and methylotrophic pathways. Thus, TE supply is critical to sustain the activity of the versatile high-performance methanogen Methanosarcina.

Dataset Information

Anaerobic digestion of chicken manure: Influence of trace element supplementation.

Publications

Anaerobic digestion of chicken manure: Influence of trace element supplementation.

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