Ontology highlight
ABSTRACT:
SUBMITTER: Han Q
PROVIDER: S-EPMC6999358 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Engineering in life sciences 20190908 11
Acetate formation is a disadvantage in the use of <i>Escherichia coli</i> for recombinant protein production, and many studies have focused on optimizing fermentation processes or altering metabolism to eliminate acetate accumulation. In this study, <i>E. coli</i> MEC697 (MG1655 <i>nadR nudC mazG</i>) maintained a larger pool of NAD(H) compared to the wild-type control, and also accumulated lower concentrations of acetate when grown in batch culture on glucose. In steady-state cultures, the elev ...[more]