Design of a high-efficiency synthetic system for l-asparaginase production in Bacillus subtilis.
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ABSTRACT:
SUBMITTER: Li X
PROVIDER: S-EPMC6999368 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
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Design of a high-efficiency synthetic system for l-asparaginase production in <i>Bacillus subtilis</i>.
Li Xu X Xu Shuqin S Zhang Xian X Xu Meijuan M Yang Taowei T Wang Li L Zhang Huiling H Fang Haitian H Osire Tolbert T Yang Shangtian S Rao Zhiming Z
Engineering in life sciences 20190213 3
l-asparaginase has high application value in medicine and food industry, but the low yield limits its application. In this study, we designed a synthetic system in <i>Bacillus subtilis</i> to produce l-asparaginase by improving gene expression and optimizing the fermentation agitation speed. Gene expression was improved by respectively increasing transcription levels and translation speeds through screening promoters and RBS sequences. With the optimal promoter, P43, and the synthetic RBS sequen ...[more]
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