Dataset Information

Rosmarinic acid-loaded electrospun nanofibers: In vitro release kinetic study and bioactivity assessment.

ABSTRACT: This study seeks to develop a nanofibrous matrix containing rosmarinic acid (RosA), an herbal non-steroidal anti-inflammatory and antioxidant drug with low water solubility, for drug delivery applications. Neat and two types of RosA-loaded cellulose acetate (CA) mats varying in the initial content of RosA were electrospun. Microstructure of nanofibers, chemistry and physical state of RosA in nanofibers, RosA loading efficiency and RosA release in acetate buffer were investigated. To evaluate bioactivity of RosA-loaded nanofibers, their ability to inhibit protein denaturation was assayed as an indicator of anti-inflammatory properties and their antioxidant activity was determined by radical scavenging assay. The indirect cytotoxicity assay was used to find if there is a cytotoxic response to nanofibers. The homogeneous distribution of the drug within nanofibers through electrospinning led to high loading efficiency, low burst release and prolonged release of a large percentage of RosA over a period of 64h following Fickian diffusion mechanism. Nanofibers with higher RosA content exhibited anti-inflammatory activity comparable to ibuprofen, and higher antioxidant activity compared to nanofibers with lower RosA content. Additionally, extracts from nanofibers did not give any major harmful effect on cells. Sustained release of RosA, and bioactivity of RosA-loaded nanofibers confirmed the potential of the produced matrix as a drug delivery system.

SUBMITTER: Vatankhah E

PROVIDER: S-EPMC6999554 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Rosmarinic acid-loaded electrospun nanofibers: In vitro release kinetic study and bioactivity assessment.

Vatankhah Elham E

Engineering in life sciences 20180925 10

This study seeks to develop a nanofibrous matrix containing rosmarinic acid (RosA), an herbal non-steroidal anti-inflammatory and antioxidant drug with low water solubility, for drug delivery applications. Neat and two types of RosA-loaded cellulose acetate (CA) mats varying in the initial content of RosA were electrospun. Microstructure of nanofibers, chemistry and physical state of RosA in nanofibers, RosA loading efficiency and RosA release in acetate buffer were investigated. To evaluate bio ...[more]

PMID: 32624867

Similar Datasets

Project description:Functional and stimuli-responsive nanofibers with an enhanced surface area/volume ratio provide controlled and triggered drug release with higher efficacy. In this study, chemotherapeutic agent Rose Bengal (RB) (4,5,6,7-tetrachloro-2', 4',5',7'-tetraiodofluoresceindisodium)-loaded water-soluble polyvinyl alcohol (PVA) nanofibers were synthesized by using the electrospinning method. A thin layer of poly(4-vinylpyridine-co-ethylene glycol dimethacrylate) p(4VP-co-EGDMA) was deposited on the RB-loaded nanofibers (PVA-RB) via initiated chemical vapor deposition (iCVD), coating the fiber surfaces to provide controllable solubility and pH response to the nanofibers. The uncoated and [p(4VP-co-EGDMA)-PVA] coated PVA-RB nanofiber mats were studied at different pH values to analyze their degradation and drug release profiles. The coated nanofibers demonstrated high stability at neutral and basic pH values for long incubation durations of 72 h, whereas the uncoated nanofibers dissolved in <2 h. The drug release studies showed that the RB release from coated PVA-RB nanofibers was higher at neutral and basic pH values, and proportional to the pH of the solution, whereas the degradation and RB release rates from the uncoated PVA-RB nanofibers were significantly higher and did not depend on the pH of environment. Further analysis of the release kinetics using the Peppas model showed that while polymer swelling and dissolution were the dominant mechanisms for the uncoated nanofibers, for the coated nanofibers, Fickian diffusion was the dominant release mechanism. The biocompatibility and therapeutic efficiency of the coated PVA-RB nanofibers against brain cancer was investigated on glioblastoma multiforme cancer cells (U87MG). The coated PVA nanofibers were observed to be highly biocompatible, and they significantly stimulated the ROS production in cells, increasing apoptosis. These promising results confirmed the therapeutic activity of the coated PVA-RB nanofibers on brain cancer cells, and encouraged their further evaluation as drug carrier structures in brain cancer treatment.

Dataset Information

Rosmarinic acid-loaded electrospun nanofibers: In vitro release kinetic study and bioactivity assessment.

Publications

Rosmarinic acid-loaded electrospun nanofibers: In vitro release kinetic study and bioactivity assessment.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets