Ontology highlight
ABSTRACT:
SUBMITTER: Yoo JI
PROVIDER: S-EPMC7000732 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Yoo Justin I JI Daugherty Patrick S PS O'Malley Michelle A MA
Nature communications 20200204 1
Epistasis emerges when the effects of an amino acid depend on the identities of interacting residues. This phenomenon shapes fitness landscapes, which have the power to reveal evolutionary paths and inform evolution of desired functions. However, there is a need for easily implemented, high-throughput methods to capture epistasis particularly at distal sites. Here, we combine deep mutational scanning (DMS) with a straightforward data processing step to bridge reads in distal sites within genes ( ...[more]