Dataset Information

High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System.

ABSTRACT: A considerable limitation of current small-animal PET/CT imaging is the low throughput of acquisitions. Consequently, to sufficiently power a study, high costs accumulate. Together with a commercial scanner manufacturer, we developed a 4-bed mouse "hotel" to simultaneously image up to 4 mice, thereby reducing costs and maximizing the efficiency of radiotracer use when compared with scans performed with a single mouse bed. Methods: For physiologic evaluation of the mouse hotel, temperature and anesthesia were tested for uniformity in conjunction with ¹⁸F-FDG PET/CT imaging of mini image-quality phantoms designed to fit the new imaging system. After reconstruction, National Electrical Manufacturers Association NU-4 tests examined uniformity, recovery coefficients, and spillover ratios. To evaluate the mouse hotel under standard in vivo imaging conditions, 4 mice were simultaneously scanned by dynamic ¹⁸F-FDG PET/CT over 60 min, and quantified images were compared with those acquired using a single mouse bed. Results: The mouse hotel maintained a constant temperature of 36.8°C ± 0.4°C, with anesthesia distributed evenly to each nose cone (2.9 ± 0.1 L/min). The National Electrical Manufacturers Association tests revealed values within tolerable limits for uniformity, for recovery coefficients in rods larger than 2 mm, and for spillover ratios in the nonradioactive water- and air-filled chambers. There was low variability in radiotracer uptake in all major organs for the mouse hotel versus the single mouse bed. Conclusion: Analysis of images acquired using the mouse hotel confirmed its utility to increase the throughput of small-animal PET imaging without considerable loss of image quality or quantitative precision. In comparison to a single mouse bed, the cost and time associated with each scan were substantially reduced.

SUBMITTER: Greenwood HE

PROVIDER: S-EPMC7002164 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System.

Greenwood Hannah E HE Nyitrai Zoltan Z Mocsai Gabor G Hobor Sandor S Witney Timothy H TH

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 20190913 2

A considerable limitation of current small-animal PET/CT imaging is the low throughput of acquisitions. Consequently, to sufficiently power a study, high costs accumulate. Together with a commercial scanner manufacturer, we developed a 4-bed mouse "hotel" to simultaneously image up to 4 mice, thereby reducing costs and maximizing the efficiency of radiotracer use when compared with scans performed with a single mouse bed. <b>Methods:</b> For physiologic evaluation of the mouse hotel, temperature ...[more]

PMID: 31519806

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Dataset Information

High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System.

Publications

High-Throughput PET/CT Imaging Using a Multiple-Mouse Imaging System.

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