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Pharmaceuticals in treated wastewater induce a stress response in tomato plants.

ABSTRACT: Pharmaceuticals remain in treated wastewater used to irrigate agricultural crops. Their effect on terrestrial plants is practically unknown. Here we tested whether these compounds can be considered as plant stress inducers. Several features characterize the general stress response in plants: production of reactive oxygen species acting as stress-response signals, MAPKs signaling cascade inducing expression of defense genes, heat shock proteins preventing protein denaturation and degradation, and amino acids playing signaling roles and involved in osmoregulation. Tomato seedlings bathing in a cocktail of pharmaceuticals (Carbamazepine, Valporic acid, Phenytoin, Diazepam, Lamotrigine) or in Carbamazepine alone, at different concentrations and during different time-periods, were used to study the patterns of stress-related markers. The accumulation of the stress-related biomarkers in leaf and root tissues pointed to a cumulative stress response, mobilizing the cell protection machinery to avoid metabolic modifications and to restore homeostasis. The described approach is suitable for the investigation of stress response of different crop plants to various contaminants present in treated wastewater.

SUBMITTER: Gorovits R

PROVIDER: S-EPMC7002738 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Pharmaceuticals in treated wastewater induce a stress response in tomato plants.

Gorovits Rena R Sobol Iris I Akama Kazuhito K Chefetz Benny B Czosnek Henryk H

Scientific reports 20200205 1

Pharmaceuticals remain in treated wastewater used to irrigate agricultural crops. Their effect on terrestrial plants is practically unknown. Here we tested whether these compounds can be considered as plant stress inducers. Several features characterize the general stress response in plants: production of reactive oxygen species acting as stress-response signals, MAPKs signaling cascade inducing expression of defense genes, heat shock proteins preventing protein denaturation and degradation, and ...[more]

PMID: 32024917

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Project description:We examined whether exposure to municipal wastewater effluent (MWWE) compromised the stress performance of laboratory-reared fathead minnows (Pimephales promelas) in a field setting. Adult minnows were caged at two sites upstream and three sites downstream of wastewater treatments plants (WWTPs) discharging MWWE into the Bow River, Calgary, Alberta, Canada. At each site one group of fish was sampled after a 26 day exposure to MWWE, while another group was subjected to 1-min air exposure followed by 60-min confinement and then sampled. Fish morphometrics and proximate composition were measured, and whole-body cortisol, glucose and lactate levels assessed as markers of the stress response. The whole-body protein, glycogen and lipid content were higher at the site closest to a WWTP outfall relative to the other downstream and upstream sites. There were no significant differences in whole-body cortisol levels in minnows sampled at sites either upstream or downstream of WWTPs. Acute stressor exposure significantly elevated whole-body cortisol levels in all groups, and this response was not modified by the location of the sampling sites. The whole-body metabolite profile, including glucose and lactate levels, were significantly higher in fish caged immediately downstream from WWTP inputs relative to upstream sites. There was an acute-stressor-mediated increase in whole-body lactate, but not glucose, levels and this response was independent of sampling site. The results reveal that the capacity to evoke an acute stress response was not compromised in fathead minnows caged for 26 days downstream of WWTPs in the Bow River. However, there were changes in the whole-body proximate composition and metabolite levels immediately downstream from the WWTP outfall suggesting greater accumulation of energy stores in these fish. Taken together, our results suggest that environmental factors in addition to contaminants, including higher water temperature and nutrient availability, influence the impact of MWWEs on fish stress performance.

Dataset Information

Pharmaceuticals in treated wastewater induce a stress response in tomato plants.

Publications

Pharmaceuticals in treated wastewater induce a stress response in tomato plants.

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