Ontology highlight
ABSTRACT:
SUBMITTER: Chen CL
PROVIDER: S-EPMC7003079 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Chen Chiao-Lin CL Rodiger Jonathan J Chung Verena V Viswanatha Raghuvir R Mohr Stephanie E SE Hu Yanhui Y Perrimon Norbert N
G3 (Bethesda, Md.) 20200206 2
CRISPR-Cas9 is a powerful genome editing technology in which a single guide RNA (sgRNA) confers target site specificity to achieve Cas9-mediated genome editing. Numerous sgRNA design tools have been developed based on reference genomes for humans and model organisms. However, existing resources are not optimal as genetic mutations or single nucleotide polymorphisms (SNPs) within the targeting region affect the efficiency of CRISPR-based approaches by interfering with guide-target complementarity ...[more]