Dataset Information

Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle.

ABSTRACT: Control of bovine viral diarrhea virus (BVDV) relies on resource-intensive sampling to detect and remove persistently infected (PI) cattle. Herd-level surveillance tools would be useful for herds with unknown BVDV status and for monitoring herds with BVDV-free status. Our objective was to determine the feasibility of using stable flies as a sampling tool to detect BVDV at the herd level. Stable flies (Stomoxys calcitrans) were fed citrated blood from either BVDV-PI or BVDV-free cattle to establish pools of 100 flies with various proportions of BVDV-fed flies (0%, 1%, 10%, 20%, 40%, or 100% in each pool). BVDV-fed flies in these pools were harvested either 1, 2, or 3?d after consuming BVDV-PI blood to determine the impact of time after feeding. Two replicates of a 3-d by 6-dilution level matrix were produced. BVDV RNA was consistently detected on day 1 when ?10% of the flies in the pool consumed PI blood. On days 2 and 3, positive BVDV RNA detection was variable and became less consistent. Our results demonstrate that BVDV RNA can be detected in stable flies after feeding on blood from PI cattle. Successful use of stable flies as a surveillance tool will require validation under field conditions.

SUBMITTER: Carlson JM

PROVIDER: S-EPMC7003224 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Publications

Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle.

Carlson Jaden M JM Vander Ley Brian L BL Lee Sang I SI Grotelueschen Dale M DM Walz Paul H PH Workman Aspen M AM Heaton Michael P MP Boxler David J DJ

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20200122 1

Control of bovine viral diarrhea virus (BVDV) relies on resource-intensive sampling to detect and remove persistently infected (PI) cattle. Herd-level surveillance tools would be useful for herds with unknown BVDV status and for monitoring herds with BVDV-free status. Our objective was to determine the feasibility of using stable flies as a sampling tool to detect BVDV at the herd level. Stable flies (<i>Stomoxys calcitrans</i>) were fed citrated blood from either BVDV-PI or BVDV-free cattle to ...[more]

PMID: 31967528

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Project description:Background and Aim:A previous study divided Indonesian bovine viral diarrhea virus (BVDV)-1 into subgenotypes BVDV-1a to BVDV-1d based on the partial NS5B gene using strain Bega as reference for BVDV-1a. In fact, it is clustered into BVDV-1c with strain Bega-like Australia. BVDV genotyping has been done on isolates from Jakarta, West and Central Java, but East Java isolates have not been genotyped. This study aimed to analyze genetic variability and amino acid residues in the nucleotide-binding pocket of the NS5B gene from infected cattle. Materials and Methods:Samples were obtained from the Sera Bank originating from active and passive surveillance of cattle that had been tested for BVDV antigen from 2013 to 2017. Detection of the p80 antibody and BVDV genotyping was carried out using ELISA and nested-multiplex-polymerase chain reaction (PCR), respectively. We defined 15 nested PCR products for partial sequencing of NS5B. Those field samples were selected from each location and year using proportional calculation as a representative sample. Homological and phylogenetic analyses of the partial NS5B gene were performed using BLAST and MEGA version 6. Results:Based on the phylogenetic tree analysis using 360 nucleotides as the partial NS5B gene, Indonesian BVDV-1 isolates from Central and East Java were subdivided to BVDV-1a (n=9), BVDV-1b (n=1), and BVDV-1c (n=5). In the present study, the homology of BVDV subgenotype -1a, -1b, and -1c was compared to the BVDV GenBank data and found 90-93%, 93%, and 92-95% respectively with the average pairwise distance of 0.207. A point mutation was shown at R283K of all BVDV isolates based on the sequence of three amino acid residues R283, R285, and I287 in the nucleotide-binding pocket as a part of the encoded RNA-dependent RNA polymerase. Conclusion:This study revealed the genetic variability of BVDV infecting cattle in Central Java and East Java, Indonesia, the subtypes BVDV-1a, BVDV-1b, BVDV-1c, and a point mutation at the R283K residue.

Dataset Information

Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle.

Publications

Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle.

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