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Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon.


ABSTRACT: Desmozoon lepeophtherii is a microsporidian associated with gill disease in farmed Atlantic salmon (Salmo salar). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individually or in small clusters. We compared the ability of 4 histologic methods to detect D. lepeophtherii spores in serial sections of Atlantic salmon gill tissue: hematoxylin and eosin (H&E), Gram-Twort (GT), calcofluor white (CW), and immunohistochemistry (IHC). Using CW as a benchmark to calculate a relative ratio, IHC consistently detected more spores than CW (median: 1.3), followed by GT (median: 0.2) and H&E (median: 0.1). IHC detected significantly more spores than GT (p?

SUBMITTER: Herrero A 

PROVIDER: S-EPMC7003232 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Comparison of histologic methods for the detection of <i>Desmozoon lepeophtherii</i> spores in the gills of Atlantic salmon.

Herrero Ana A   Padrós Francesc F   Pflaum Sara S   Matthews Chris C   Del-Pozo Jorge J   Rodger Hamish D HD   Dagleish Mark P MP   Thompson Kim D KD  

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20191118 1


<i>Desmozoon lepeophtherii</i> is a microsporidian associated with gill disease in farmed Atlantic salmon (<i>Salmo salar</i>). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individually or in small clusters. We compared the ability of 4 histologic methods to detect <i>D. lepeophtherii</i> spores in serial sections of Atlantic salmon gill tissue: hematoxylin and eos  ...[more]

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