Intervertebral disc degeneration in mice with type II diabetes induced by leptin receptor deficiency.

ABSTRACT: BACKGROUND:The leptin receptor-deficient knockout (db/db) mouse is a well-established model for studying type II diabetes mellitus (T2DM). T2DM is an important risk factor of intervertebral disc degeneration (IVDD). Although the relationship between type I diabetes and IVDD has been reported by many studies, few studies have reported the effects of T2DM on IVDD in db/db mice model. METHODS:Mice were separated into 3 groups: wild-type (WT), db/db, and IGF-1 groups (leptin receptor-deficient mice were treated with insulin-like growth factor-1 (IGF-1). To observe the effects of T2DM and glucose-lowering treatment on IVDD, IGF-1 injection was used. The IVD phenotype was detected by H&E and safranin O fast green staining among db/db, WT and IGF-1 mice. The levels of blood glucose and weight in mice were also recorded. The changes in the mass of the trabecular bone in the fifth lumbar vertebra were documented by micro-computed tomography (micro-CT). Tunnel assays were used to detect cell apoptosis in each group. RESULTS:The weight of the mice were 27.68?±?1.6?g in WT group, which was less than 57.56?±?4.8?g in db/db group, and 52.17?±?3.7?g in IGF-1 injected group (P?

SUBMITTER: Li X 

PROVIDER: S-EPMC7003448 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature