Ontology highlight
ABSTRACT:
SUBMITTER: de Boer OJ
PROVIDER: S-EPMC7003497 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
de Boer Onno J OJ Krebbers Gabrielle G Mackaaij Claire C Florquin Sandrine S de Rie Menno A MA van der Wal Allard C AC Teunissen Marcel B M MBM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 20191227 2
Lack of specific markers for innate lymphoid cells (ILCs) limit our knowledge on their spatial organization in situ. We compared two quadruple-color staining protocols for detection of the three principal human ILC subsets in formalin-fixed paraffin-embedded specimens. ILC subset-associated archetypical transcription factors (TFs) T-bet, GATA3, and RORγt were used as positive identifiers in combination with lymphoid lineage markers to exclude non-ILCs. One method ("virtual quadruple staining") c ...[more]